Measurement of red cell folate with 75Se-selenofolate radioassay.
نویسندگان
چکیده
75Se-selenofolate radioassay was adapted for the purpose of red cell folate measurement. The results of assay of 124 whole blood samples by Lactobacillus casei microbiological assay and by radioassay using the adapted selenofolate method were concordant (r = 0.8). Red cell folate levels by the radioassay were below 170 microgram/1 in people who had haematological features of folate depletion. The distribution of results among reputedly healthy subjects extended down to 140 microgram/1.
منابع مشابه
Analytical evaluation of a folate radioassay: application to serum and erythrocyte measurements.
Many commercial folate radioassay kits have proven to be unsatisfactory in the past. It has been found that the Schwarz/Mann Iodine-125 folate radioassay kit permits a rapid measurement of folic acid requiring only a single incubation at pH 9.3. At this pH, the relative affinity of pteroylglutamic acid (FA) and N-5-methyltetrahydrofolic acid (MTFA) for the milk binder is the same. Additionally,...
متن کاملComparison of radioassay and microbiological assay for serum folate, with clinical assessment of discrepant results.
Folate assays by use of radiolabeled folate provide obvious practical advantages over the standard microbiological assay, but remain incompletely tested. We therefore compared results for 415 sera with a kit involving 3H-labeled folate and the Lactobacillus casei microbiological method. We examined the patients' data when there were discrepancies between the two methods. Although the correlatio...
متن کاملAssessment of the value of a competitive protein binding radioassay of folic acid in the detection of folic acid deficiency.
The diagnostic value of the Becton Dickinson Radioassay Kit (125I) for the the assay of red cell folate has been investigated. The assay was acceptable with regards to precision but was non-linear with changing packed cell volume. Sensitivity of the assay was satisfactory, with 24 of 25 folate deficient patients giving red cell folate values which fell below the reference range. Specificity of ...
متن کاملEffect of different dosage and administration schedules of folic acid on blood folate levels in a population of Honduran women of reproductive age.
BACKGROUND Observational studies and clinical trials have shown conclusive evidence that periconceptional folic acid supplementation prevents up to 70 % of neural tube defects (NTD). The Honduran government wanted to implement a supplementation programme of folic acid but needed to assess the relative effects of two dosages of folic acid. OBJECTIVE To determine the effect of two dosages of fo...
متن کاملLaboratory diagnosis of megaloblastic anaemia: current methods assessed by external quality assurance trials.
The results of an Interregional quality assurance scheme for tests in the diagnosis of megaloblastic anaemia were reviewed to assess the methods used. Serum folate assays showed great variation between methods, partly due to limitations in assessment by external quality assurance. Red cell folate assays yielded widely different results and much imprecision due both to the differences in prepara...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 31 1 شماره
صفحات -
تاریخ انتشار 1978